| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Nguyen, Nam Trung
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (3/3 displayed)
- 2022Plasma-Induced Nanocrystalline Domain Engineering and Surface Passivation in Mesoporous Chalcogenide Semiconductor Thin Filmscitations
- 2018Robust free-standing nano-thin SiC membranes enable direct photolithography for MEMS sensing applicationscitations
- 2018Graphene-oxide-loaded superparamagnetic iron oxide nanoparticles for ultrasensitive electrocatalytic detection of microRNAcitations
Places of action
| Organizations | Location | People |
|---|
article
Graphene-oxide-loaded superparamagnetic iron oxide nanoparticles for ultrasensitive electrocatalytic detection of microRNA
Abstract
We report the electrocatalytic activity of a new class of superparamagnetic nanoparticles, graphene-oxide-loaded iron oxide (GO/IO hybrid material), towards the reduction of ruthenium hexaammine(III) chloride (Ru(NH3)6]<sup>3+</sup>, RuHex). Leveraging the electrocatalytic activity of the GO/IO hybrid material and the signal enhancement capacity of [Ru(NH<sub>3</sub>)<sub>6</sub>]<sup>3+</sup>/[Fe(CN)<sub>6</sub>]<sup>3−</sup> in an electrocatalytic cycle, an ultrasensitive and specific electrochemical sensor was developed for the detection of cancer-related microRNA (miRNA). Using the direct affinity interaction between RNA and graphene oxide, magnetically isolated and purified target miRNA were directly adsorbed onto a screen-printed electrode modified with the GO/IO hybrid material. The detection was enabled by chronocoulometric (CC) readout of charge-compensating [Ru(NH<sub>3</sub>)<sub>6</sub>]<sup>3+</sup> followed by an enhancement in CC charge display through the Ru(NH<sub>3</sub>)<sub>6</sub>]<sup>3+</sup>/[Fe(CN)<sub>6</sub>]<sup>3− </sup>system. We demonstrate an excellent limit of detection of 1.0 fM by accurately detecting miR-21 in synthetic samples and showcase its clinical utility in ovarian cancer cell lines with high sensitivity (ten cells) and good reproducibility (% RSD=<5 %, for <i>n</i>=3).