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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Estrela, Pedro
University of Bath
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (17/17 displayed)
- 2021CRISPR-based electrochemical sensor for COVID-19 diagnostics
- 2021Graphene enabled low-noise surface chemistry for multiplexed sepsis biomarker detection in whole bloodcitations
- 2020Electrochemical ELISA Protein Biosensing in Undiluted Serum Using a Polypyrrole-Based Platformcitations
- 2019A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantificationcitations
- 2019Reduced Graphene-Oxide Transducers for Biosensing Applications Beyond the Debye-Screening Limitcitations
- 2019In-situ synthesis of 3D ultra-small gold augmented graphene hybrid for highly sensitive electrochemical binding capabilitycitations
- 2019Multiplexed Electrochemical Platform for sepsis Diagnostics
- 2017Raman and Mössbauer spectroscopic studies of tungsten doped Ni–Zn nano ferritecitations
- 2017Nanomaterial fungicides: In vitro and in vivo antimycotic activity of cobalt and nickel nanoferrites on phytopathogenic fungicitations
- 2016Electrochemical biosensors and nanobiosensorscitations
- 2016Inexpensive and fast pathogenic bacteria screening using field-effect transistorscitations
- 2016Inexpensive and fast pathogenic bacteria screening using field-effect transistorscitations
- 2016Aptamer-MIP hybrid receptor for highly sensitive electrochemical detection of prostate specific antigencitations
- 2011Room temperature processed ISFETs based on amorphous semiconductors oxides
- 2002High pressure transport study of non-Fermi liquid behaviour in U2Pt2In and U3Ni3Sn4
- 2002Pressure-induced recovery of the Fermi-liquid state in the non-Fermi liquid material U2Pt2Incitations
- 2000Possible non-Fermi-liquid behaviour in URh1/3Ni2/3Alcitations
Places of action
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article
Graphene enabled low-noise surface chemistry for multiplexed sepsis biomarker detection in whole blood
Abstract
<p>Affinity-based electrochemical (EC) sensors offer a potentially valuable approach for point-of-care (POC) diagnostics applications, and for the detection of diseases, such as sepsis, that require simultaneous detection of multiple biomarkers, but their development has been hampered due to biological fouling and EC noise. Here, an EC sensor platform that enables detection of multiple sepsis biomarkers simultaneously by incorporating a nanocomposite coating composed of crosslinked bovine serum albumin containing a network of reduced graphene oxide nanoparticles that prevents biofouling while maintaining electroconductivity is described. Using nanocomposite coated planar gold electrodes, a sensitive procalcitonin (PCT) sensor is constructed and validated in undiluted serum, which produced an excellent correlation with a conventional ELISA (adjusted r<sup>2</sup> = 0.95) using clinical samples. A single multiplexed platform containing sensors for three different sepsis biomarkers—PCT, C-reactive protein, and pathogen-associated molecular patterns—is also developed, which exhibits specific responses within the clinically significant range without any cross-reactivity. This platform enables sensitive simultaneous EC detection of multiple analytes in human whole blood, and it can be applied to detect any target analyte with an appropriate antibody pair. Thus, this nanocomposite-enabled EC sensor platform may offer a potentially valuable tool for development of a wide range of clinical POC diagnostics.</p>