| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Lima, João Carlos
Altice Portugal (Portugal)
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (10/10 displayed)
- 2024Cryoprotective Polysaccharides with Ordered Gel Structures Induce Ice Growth Anticipation and Survival Enhancement during Cell Cryopreservationcitations
- 2020Application of polyoxometalate-ionic liquids (POM-ILs) in dye-sensitized solar cells (DSSCs)citations
- 2017A non-invasive optical method for mapping temperature polarization in direct contact membrane distillationcitations
- 2016Molecular Weight Determination by Luminescent Chemo–enzymaticscitations
- 2015Tuning the Surface Chemistry of Nanoporous Carbons for Enhanced Nanoconfined Photochemical Activitycitations
- 2013Photoinduced reactions occurring on activated carbons. A combined photooxidation and ESR studycitations
- 2008Study of the effect of the phosphane bridging chain nature on the structural and photophysical properties of a series of gold(I) ethynylpyridine complexescitations
- 2008Probing the microenvironment of sol-gel entrapped cutinase: the role of added zeolite NaYcitations
- 2006Metallodendrimers containing both ruthenium (internal layer) and rhenium (external layer)citations
- 2000Fluorescence-labeled pyrenesulfonamide response for characterizing polymeric interfaces in composite materialscitations
Places of action
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article
Cryoprotective Polysaccharides with Ordered Gel Structures Induce Ice Growth Anticipation and Survival Enhancement during Cell Cryopreservation
Abstract
This work cross-correlated rheological, thermodynamic, and conformational features of several natural polysaccharides to their cryoprotective performance. The basis of cryoprotection of FucoPol, pectin, and agar revealed a causal combination of (i) an emerging sol-gel transition (p = 0.014) at near-hypothermia (4 °C), (ii) noncolligative attenuated supercooling of the kinetic freezing point of water (p = 0.026) supporting ice growth anticipation, and (iii) increased conformational order (p < 0.0001), where helix-/sheet-like features boost cryoprotection. FucoPol, of highest cryoprotective performance, revealed a predominantly helical structure (α/β = 1.5) capable of forming a gel state at 4 °C and the highest degree of supercooling attenuation (TH = 6.2 °C). Ice growth anticipation with gel-like polysaccharides suggests that the gel matrix neutralizes elastic deformations and lethal cell volumetric fluctuations during freezing, thus preventing the loss of homeostasis and increasing post-thaw viability. Ultimately, structured gels capable of attenuated supercooling enable cryoprotective action at the polymer-cell interface, in addition to polymer-ice interactions. This rationale potentiates implementing alternative, biobased, noncytotoxic polymers in cryobiology.