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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Alegret, Salvador
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (25/25 displayed)
- 2011Magneto immunoassays for plasmodium falciparum histidine-rich protein 2 related to malaria based on magnetic nanoparticlescitations
- 2009Electrochemical immunosensor for the diagnosis of celiac diseasecitations
- 2009Double-tagging polymerase chain reaction with a thiolated primer and electrochemical genosensing based on gold nanocomposite sensor for food safetycitations
- 2009Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensorscitations
- 2007Electrochemical magneto immunosensing of antibiotic residues in milkcitations
- 2007Bioaffinity platforms based on carbon-polymer biocomposites for electrochemical biosensingcitations
- 2007Electrochemical biosensing of pesticide residues based on affinity biocomposite platformscitations
- 2007In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogenscitations
- 2006Novel routes for inter-matrix synthesis and characterization of polymer stabilized metal nanoparticles for molecular recognition devicescitations
- 2006Impedimetric genosensors for the detection of DNA hybridizationcitations
- 2006Genomagnetic assay based on label-free electrochemical detection using magneto-composite electrodescitations
- 2006Urea impedimetric biosensor based on polymer degradation onto interdigitated electrodescitations
- 2006Electrochemical magnetoimmunosensing strategy for the detection of pesticides residuescitations
- 2006Extractant assisted synthesis of polymer stabilized platinum and palladium metal nanoparticles for sensor applicationscitations
- 2006Electrochemical biosensing based on universal affinity biocomposite platformscitations
- 2005Integration of a glucose biosensor based on an epoxy-graphite- TTF·TCNQ-GOD biocomposite into a FIA systemcitations
- 2005Magnetically trigged direct electrochemical detection of DNA hybridization using Au67 quantum dot as electrical tracercitations
- 2005Electrochemical genosensing based on rigid carbon composites. A reviewcitations
- 2005Glucose biosensor based on carbon nanotube epoxy compositescitations
- 2005Sensitive stripping voltammetry of heavy metals by using a composite sensor based on a built-in bismuth precursorcitations
- 2004Renewable Protein A modified graphite-epoxy composite for electrochemical immunosensingcitations
- 2004Rigid carbon composites: A new transducing material for label-free electrochemical genosensingcitations
- 2003Graphite-epoxy platforms for electrochemical genosensingcitations
- 2003Rapid electrochemical genosensor assay using a streptavidin carbon-polymer biocomposite electrodecitations
- 2003Graphite-epoxy composites as a new transducing material for electrochemical genosensingcitations
Places of action
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article
In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogens
Abstract
A sensitive and selective genomagnetic assay for the electrochemical detection of food pathogens based on in situ DNA amplification with magnetic primers has been designed. The performance of the genomagnetic assay was firstly demonstrated for a DNA synthetic target by its double-hybridization with both a digoxigenin probe and a biotinylated capture probe, and further binding to streptavidin-modified magnetic beads. The DNA sandwiched target bound on the magnetic beads is then separated by using a magneto electrode based on graphite-epoxy composite. The electrochemical detection is finally achieved by an enzyme marker, anti-digoxigenin horseradish peroxidase (HRP). The novel strategy was used for the rapid and sensitive detection of polymerase chain reaction (PCR) amplified samples. Promising resultants were also achieved for the DNA amplification directly performed on magnetic beads by using a novel magnetic primer, i.e., the up PCR primer bound to magnetic beads. Moreover, the magneto DNA biosensing assay was able to detect changes at single nucleotide polymorphism (SNP) level, when stringent hybridization conditions were used. The reliability of the assay was tested for Salmonella spp., the most important pathogen affecting food safety. © 2006 Elsevier B.V. All rights reserved.