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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Gil, F. J.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (35/35 displayed)
- 2019Control of stem cell response and bone growth on biomaterials by fully non-peptidic integrin selective ligandscitations
- 2017Functionalization of CoCr surfaces with cell adhesive peptides to promote HUVECs adhesion and proliferationcitations
- 2017Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosiscitations
- 2015Development of tantalum scaffold for orthopedic applications produced by space-holder methodcitations
- 2014Biomimetic treatment on dental implants for short-term bone regenerationcitations
- 2012Variation of the superelastic properties and nickel release from original and reused NiTi orthodontic archwirescitations
- 2011Reduction of Ni release and improvement of the friction behaviour of NiTi orthodontic archwires by oxidation treatmentscitations
- 2011Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titaniumcitations
- 2010Development of provisional extracellular matrix on biomaterials interface: Lessons from in vitro cell culturecitations
- 2009Influence of Cold Work in the Elastic Modulus of the Ti-16.2Hf-24.8Nb-1Zr Alloy Characterized by Instrumented Nanoindentationcitations
- 2008Oxidized NiTi surfaces enhance differentiation of osteoblast-like cellscitations
- 2008Effect of the sterilization process on physical and mechanical properties of the Bonacryl bone cement
- 2008EFFECT OF THE STERILIZATION PROCESS ON PHYSICAL AND MECHANICAL PROPERTIES OF THE BONACRYL BONE CEMENT
- 2007Low elastic modulus metals for joint prosthesiscitations
- 2007Electrochemical behaviour of oxidized NiTi shape memory alloys for biomedical applicationscitations
- 2006New oxidation treatment of NiTi shape memory alloys to obtain Ni-free surfaces and to improve biocompatibilitycitations
- 2005Processing and mechanical properties of hydroxyapatite pieces obtained by the gelcasting methodcitations
- 2005Synthesis and characterization of poly (methyl methacrylate-styrene) copolymeric beads for bone cements
- 2004Propagation of fatigue cracks in acrylic bone cements containing different radiopaque agentscitations
- 2004Wear behaviour of the pair Ti-6Al-4V-UHMWPE of acrylic bone cements containing different radiopaque agentscitations
- 2004Osseointegration of Grit-Blasted and Bioactive Titanium Implants
- 2004Wollastonite coatings on zirconia ceramicscitations
- 2003The effect of cooling rate on the cyclic deformation of beta-annealed Ti-6Al-4Vcitations
- 2002Effect of oxygen content on grain growth kinetics of titaniumcitations
- 2002Acrylic-phosphate glasses composites as self-curing controlled delivery systems of antibioticscitations
- 2002Mechanical performance of acrylic bone cements containing different radiopacifying agentscitations
- 2002Growth of Bioactive Surfaces on Dental Implantscitations
- 2002Human-osteoblast proliferation and differentiation on grit-blasted and bioactive titanium for dental applicationscitations
- 2002Surface hardening by anodizing and heat treatments of Ti6A14V alloys for articular prostheses
- 2002Physical and mechanical behavior of zirconia-hydroxyapatite ceramics after aging in simulated body fluidcitations
- 2002Surface hardening by anodizing and heat treatments of Ti6Al4V alloys for articular prosthesescitations
- 2002Growth of bioactive surfaces on titanium and its alloys for orthopaedic and dental implantscitations
- 2001Formation of alpha-Widmanstatten structure: effects of grain size and cooling rate on the Widmanstatten morphologies and on the mechanical properties in Ti6Al4V alloycitations
- 2001Fatigue crack propagation of acrylic bone cements. Influence of the radio-opaque agents,Propagación de grietas por fatiga de cementos óseos acrílicos. Influencia de los agentes radiopacos
- 2001Fatigue crack propagation of acrylic bone cements. Influence of the radio-opaque agents
Places of action
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article
Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium
Abstract
<p>Biomaterial surface properties, via alterations in the adsorbed protein layer, and the presence of specific functional groups can influence integrin binding specificity, thereby modulating cell adhesion and differentiation processes. The adsorption of fibronectin, a protein directly involved in osteoblast adhesion to the extracellular matrix, has been related to different physical and chemical properties of biomaterial surfaces. This study used blasting particles of different sizes and chemical compositions to evaluate the response of MG63 osteoblast-like cells on smooth and blasted titanium surfaces, with and without fibronectin coatings, by means of real-time reverse transcription-polymerase chain reaction (qRT-PCR) assays. This response included (a) expression of the α<sub>5</sub>, α<sub>v</sub> and α<sub>3</sub>integrin subunits, which can bind to fibronectin through the RGD binding site, and (b) expression of alkaline phosphatase (ALP) and osteocalcin (OC) as cell-differentiation markers. ALP activity and synthesis of OC were also tested. Cells on SiC-blasted Ti surfaces expressed higher amounts of the α<sub>5</sub> mRNA gene than cells on Al<sub>2</sub>O <sub>3</sub>-blasted Ti surfaces. This may be related to the fact that SiC-blasted surfaces adsorbed higher amounts of fibronectin due to their higher surface free energy and therefore provided a higher number of specific cell-binding sites. Fn-coated Ti surfaces decreased α<sub>5</sub> mRNA gene expression, by favoring the formation of other integrins involved in adhesion over α<sub>5</sub>β<sub>1</sub>. The changes in a5 mRNA expression induced by the presence of fibronectin coatings may moreover influence the osteoblast differentiation pathway, as fibronectin coatings on Ti surfaces also decreased both ALP mRNA expression and ALP activity after 14 and 21 days of cell culture.</p>