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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Brzózka, Zbigniew
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (10/10 displayed)
- 20192D Ti2C (MXene) as a novel highly efficient and selective agent for photothermal therapycitations
- 2017Microfluidic system for monitoring of cardiac (H9C2) cell proliferation
- 2016Evaluation of nanoencapsulated verteporfin's cytotoxicity using a microfluidic systemcitations
- 2012Effect of a high surface-to-volume ratio on fluorescence-based assayscitations
- 2007Further studies on the role of redox-active monolayer as intermediate phase of solid-state sensorscitations
- 2006Fabrication of ceramic-polymer microstructure for capillary electrophoresis with usage of photosensitive paste and thick film technology
- 2005Studies on ferrocene organothiol monolayer as an intermediate phase of potentiometric sensors with gold inner contactcitations
- 2004Design of ferrocene organothiol monolayer as intermediate phase for miniaturized electrochemical sensors with gold contact
- 2004Towards advanced chemical microsensors—an overviewcitations
- 2001Multi-ion analysis based on versatile sensor headcitations
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article
Effect of a high surface-to-volume ratio on fluorescence-based assays
Abstract
In the work discussed in this paper, the effect of a high surface-to-volume ratio of a microfluidic detection cell on fluorescence quenching was studied. It was found that modification of the geometry of a microchannel can provide a wider linear range. This is a phenomenon which should be taken into consideration when microfluidic systems with fluorescence detection are developed. The dependence of the linear range for fluorescein on the surface-to-volume ratio was determined. Both fluorescence inner-filter effects and concentration self-quenching were taken into consideration. It was found that inner-filter effects have little effect on the extent of the linear range on the microscale. Figure Dependence of the linear range on surface-to-volume ratio in microfluidic detection.