| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Lockyer, Nicholas P.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (17/17 displayed)
- 2023A high-resolution versatile focused ion implantation platform for nanoscale engineeringcitations
- 2016Evaluation of biomolecular distributions in rat brain tissues by means of ToF-SIMS using a continuous beam of Ar clusterscitations
- 2015Mass spectrometric imaging of brain tissue by time-of-flight secondary ion mass spectrometry - How do polyatomic primary beams C 60 + , Ar 2000 + , water-doped Ar 2000 + and (H 2 O) 6000 + compare?citations
- 2015Mass spectrometric imaging of brain tissue by time-of-flight secondary ion mass spectrometry – How do polyatomic primary beams C60+, Ar2000+, water-doped Ar2000+ and (H2O)6000+ compare?citations
- 2013Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancercitations
- 2013Peptide structural analysis using continuous Ar cluster and C60 ion beamscitations
- 2013Peptide structural analysis using continuous Ar cluster and C60 ion beamscitations
- 2013Peak picking as a pre-processing technique for imaging time of flight secondary ion mass spectrometrycitations
- 2013ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systemscitations
- 2012Peak picking as a pre-processing technique for imaging time of flight secondary ion mass spectrometry
- 2011Three-dimensional mass spectral imaging of HeLa-M cells - Sample preparation, data interpretation and visualisationcitations
- 2010Influence of omega-6 PUFA arachidonic acid and bone marrow adipocytes on metastatic spread from prostate cancercitations
- 2010Effects of cryogenic sample analysis on molecular depth profiles with TOF-secondary ion mass spectrometrycitations
- 2008Subsurface biomolecular imaging of Streptomyces coelicolor using secondary ion mass spectrometrycitations
- 2008Discrimination of prostate cancer cells and non-malignant cells using secondary ion mass spectrometrycitations
- 2008A new dynamic in mass spectral imaging of single biological cellscitations
- 2004The combined application of FTIR microspectroscopy and ToF-SIMS imaging in the study of prostate cancercitations
Places of action
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article
Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer
Abstract
It is known that hypoxia-inducible factor 1 (HIF-1) activity results in the coordinated up-regulation of a large number of proteins that facilitate cell survival in tumours; however, the effect of HIF-1 on cancer metabolism is less well characterised. With knowledge of the specific effect of HIF-1 on cancer metabolism, biomarkers could be identified for which new drugs could be targeted. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) offers the potential to analyse intact cells in situ and has a mass spectral coverage that is applicable to metabolic profiling. It has been used to analyse the effects of HIF-1 on multicellular tumour models. Multicellular tumour spheroids (MTSs) have been cultured from human colon carcinoma cells with and without the expression of HIF-1, and the surface of the cross sections of each MTS has been analysed. Because metabolic profiling is an emerging field in ToF-SIMS, there is a requirement to determine which metabolites can be detected using this technique and which of those can be identified in complex mixtures within biological samples. For this, a selection of metabolites have been analysed, and the ToF-SIMS standard spectra acquired have been used to localise metabolites in MTS sections. The comparison of metabolic profiles of MTSs with and without the expression of HIF-1 has elucidated potential biomarkers for tumour survival in hypoxia, some of which may be HIF-1 regulated. Copyright © 2012 John Wiley & Sons, Ltd. Copyright © 2012 John Wiley & Sons, Ltd.